Generated:Article Title: Inducible TDG knockout models to study epigenetic regulation
Article Snippet: .. Tdgtm1Psch mice (http://www.informatics.jax.org/allele/MGI:5487834) were generated from E14 ESC (RRID:CVCL_C320) and backcrossed for more than 10 generations with female C57BL/ 6JRj mice, obtained at 7-8 weeks of age from Janvier Labs. A Tdg expression cassette (pTCO2-mTDGi.0, Addgene Plasmid #149429) was introduced as single copy into C57BL/6JRj mice by pronuclear injection and crossed into Tdgtm1Psch mice. .. Genotyping was done by PCR with the HOT FIREPol (Solis BioDyne) on diluted crude DNA preparations (diluted by a factor of five with 10 mM Tris-HCl pH8) by boiling toe clips with 25 mM NaOH/0.2 mM EDTA for 1 h followed by neutralization with 40 mM Tris-HCl.
Expressing:Article Title: Inducible TDG knockout models to study epigenetic regulation
Article Snippet: .. Tdgtm1Psch mice (http://www.informatics.jax.org/allele/MGI:5487834) were generated from E14 ESC (RRID:CVCL_C320) and backcrossed for more than 10 generations with female C57BL/ 6JRj mice, obtained at 7-8 weeks of age from Janvier Labs. A Tdg expression cassette (pTCO2-mTDGi.0, Addgene Plasmid #149429) was introduced as single copy into C57BL/6JRj mice by pronuclear injection and crossed into Tdgtm1Psch mice. .. Genotyping was done by PCR with the HOT FIREPol (Solis BioDyne) on diluted crude DNA preparations (diluted by a factor of five with 10 mM Tris-HCl pH8) by boiling toe clips with 25 mM NaOH/0.2 mM EDTA for 1 h followed by neutralization with 40 mM Tris-HCl.
Plasmid Preparation:Article Title: Inducible TDG knockout models to study epigenetic regulation
Article Snippet: .. Tdgtm1Psch mice (http://www.informatics.jax.org/allele/MGI:5487834) were generated from E14 ESC (RRID:CVCL_C320) and backcrossed for more than 10 generations with female C57BL/ 6JRj mice, obtained at 7-8 weeks of age from Janvier Labs. A Tdg expression cassette (pTCO2-mTDGi.0, Addgene Plasmid #149429) was introduced as single copy into C57BL/6JRj mice by pronuclear injection and crossed into Tdgtm1Psch mice. .. Genotyping was done by PCR with the HOT FIREPol (Solis BioDyne) on diluted crude DNA preparations (diluted by a factor of five with 10 mM Tris-HCl pH8) by boiling toe clips with 25 mM NaOH/0.2 mM EDTA for 1 h followed by neutralization with 40 mM Tris-HCl.
Injection:Article Title: Inducible TDG knockout models to study epigenetic regulation
Article Snippet: .. Tdgtm1Psch mice (http://www.informatics.jax.org/allele/MGI:5487834) were generated from E14 ESC (RRID:CVCL_C320) and backcrossed for more than 10 generations with female C57BL/ 6JRj mice, obtained at 7-8 weeks of age from Janvier Labs. A Tdg expression cassette (pTCO2-mTDGi.0, Addgene Plasmid #149429) was introduced as single copy into C57BL/6JRj mice by pronuclear injection and crossed into Tdgtm1Psch mice. .. Genotyping was done by PCR with the HOT FIREPol (Solis BioDyne) on diluted crude DNA preparations (diluted by a factor of five with 10 mM Tris-HCl pH8) by boiling toe clips with 25 mM NaOH/0.2 mM EDTA for 1 h followed by neutralization with 40 mM Tris-HCl.
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